Pair Name	Thymoquinone, Gemcitabine
Disease Info	[ICD-11: 2C10]	Pancreatic cancer		Investigative
Biological Phenomena	Induction-->Apoptosis
Gene Regulation	Down-regulation	Expression	COL1A1	hsa1277
	Down-regulation	Expression	COL3A1	hsa1281
	Down-regulation	Expression	COL5A1	hsa1289
	Down-regulation	Expression	HIF1A	hsa3091
	Down-regulation	Expression	SMAD2	hsa4087
	Down-regulation	Expression	SMAD3	hsa4088
	Down-regulation	Expression	TGFB1	hsa7040
In Vitro Model	PANC-1	Pancreatic ductal adenocarcinoma	Homo sapiens (Human)	CVCL_0480
In Vivo Model	PANC-1 cells cultured under normoxic conditions formed subcutaneous tumors in nude mice, with a tumorigenic cycle of 4 weeks (grouping: a total of 9 mice were divided into three groups with 3 mice per group; age: 6– weeks; weight: 200–220 g).
Result	TQ can promote apoptosis, inhibit migration, invasion, and metastasis, and enhance the sensitivity to GEM. The underlying mechanism may involve the regulation of ECM production through the TGFβ/Smad pathway, in which HIF-1α plays a key role.